proBDNF, a precursor of brain-derived neurotrophic factor (BDNF), is anterogradely
transported and released from nerve terminals, but the mechanism underlying this process
remains unclear. In this study, we report that proBDNF forms a complex with Huntingtin
associated protein-1 (HAP1) and sortilin, which plays an important role in proBDNF
intracellular trafficking and stabilization. The interaction of proBDNF with both HAP1A and
sortilin in co-transfected HEK293 cells is confirmed by both fluorescence resonance energy …