[HTML][HTML] VEGF-C, a lymphatic growth factor, is a RANKL target gene in osteoclasts that enhances osteoclastic bone resorption through an autocrine mechanism
Q Zhang, R Guo, Y Lu, L Zhao, Q Zhou… - Journal of biological …, 2008 - ASBMB
Osteoclasts are bone-resorbing cells, but they also secrete and respond to cytokines. Here,
we test the hypothesis that osteoclasts secrete the lymphatic growth factor, VEGF-C, to
increase their resorptive activity. Osteoclasts and osteoclast precursors were generated by
culturing splenocytes with macrophage colony-stimulating factor and RANKL from wild-type,
NF-κBp50-/-/p52-/-, and Src-/-mice. Expression of VEGFs was measured by real time reverse
transcription-PCR, Western blotting, and immunostaining. The effect of VEGF-C signaling on …
we test the hypothesis that osteoclasts secrete the lymphatic growth factor, VEGF-C, to
increase their resorptive activity. Osteoclasts and osteoclast precursors were generated by
culturing splenocytes with macrophage colony-stimulating factor and RANKL from wild-type,
NF-κBp50-/-/p52-/-, and Src-/-mice. Expression of VEGFs was measured by real time reverse
transcription-PCR, Western blotting, and immunostaining. The effect of VEGF-C signaling on …