Steady‐state kinetic tracer analysis and two‐dimensional chromatography and autoradiography were used to examine the relation of glucose transport to its metabolism in cultured chick embryo fibroblasts. Cytochalasin B was added to Rous sarcoma virus‐infected cells to bring the rate of glucose uptake to the level of uninfected cells. It is concluded for chick cells in culture that: (1) the transport of glucose, rather than its phosphorylation, is the rate limiting step and (2) the difference in aerobic glycolysis between normal and virus‐transformed cells at physiological glucose concentration may be a consequence of the difference in the rates of glucose uptake.