NSAIDs acutely inhibit TRPC channels in freshly isolated rat glomeruli
DV Ilatovskaya, V Levchenko, RP Ryan… - Biochemical and …, 2011 - Elsevier
DV Ilatovskaya, V Levchenko, RP Ryan, AW Cowley Jr, A Staruschenko
Biochemical and biophysical research communications, 2011•ElsevierUsing a novel approach for analysis of TRPC channel activity, we report here that NSAIDs
are involved into regulation of TRPC channels in the podocytes of the freshly isolated
decapsulated glomeruli. Fluorescence and electron microscopy techniques confirmed the
integrity of podocytes in the glomeruli. Western blotting showed that TRPC1, 3 and 6 are
highly expressed in the glomeruli. Single-channel patch clamp analysis revealed cation
currents with distinct TRPC properties. This is the first report describing single TRPC-like …
are involved into regulation of TRPC channels in the podocytes of the freshly isolated
decapsulated glomeruli. Fluorescence and electron microscopy techniques confirmed the
integrity of podocytes in the glomeruli. Western blotting showed that TRPC1, 3 and 6 are
highly expressed in the glomeruli. Single-channel patch clamp analysis revealed cation
currents with distinct TRPC properties. This is the first report describing single TRPC-like …
Abstract
Using a novel approach for analysis of TRPC channel activity, we report here that NSAIDs are involved into regulation of TRPC channels in the podocytes of the freshly isolated decapsulated glomeruli. Fluorescence and electron microscopy techniques confirmed the integrity of podocytes in the glomeruli. Western blotting showed that TRPC1, 3 and 6 are highly expressed in the glomeruli. Single-channel patch clamp analysis revealed cation currents with distinct TRPC properties. This is the first report describing single TRPC-like currents in glomerular podocytes. Furthermore, our data provide a novel mechanism of NSAIDs regulation of TRPC channels, which might be implicated in maintaining the glomerular filtration barrier.
Elsevier