Decreased ratio of CD8+ T cells to regulatory T cells associated with decreased survival in dogs with osteosarcoma
BJ Biller, A Guth, JH Burton… - Journal of veterinary …, 2010 - Wiley Online Library
BJ Biller, A Guth, JH Burton, SW Dow
Journal of veterinary internal medicine, 2010•Wiley Online LibraryBackground: Increased numbers of regulatory T cells (Treg) and decreased ratios of CD8+ T
cells to Treg have been shown to correlate with decreased survival times (ST) in humans
with certain malignancies. A possible connection between Treg and ST in dogs with cancer
has not been investigated previously. Hypothesis: The purpose of this study was to compare
numbers of Treg and T lymphocyte subsets in dogs with osteosarcoma (OSA) to those of
healthy dogs and to determine whether pretreatment values were associated with disease …
cells to Treg have been shown to correlate with decreased survival times (ST) in humans
with certain malignancies. A possible connection between Treg and ST in dogs with cancer
has not been investigated previously. Hypothesis: The purpose of this study was to compare
numbers of Treg and T lymphocyte subsets in dogs with osteosarcoma (OSA) to those of
healthy dogs and to determine whether pretreatment values were associated with disease …
Background: Increased numbers of regulatory T cells (Treg) and decreased ratios of CD8+ T cells to Treg have been shown to correlate with decreased survival times (ST) in humans with certain malignancies. A possible connection between Treg and ST in dogs with cancer has not been investigated previously.
Hypothesis: The purpose of this study was to compare numbers of Treg and T lymphocyte subsets in dogs with osteosarcoma (OSA) to those of healthy dogs and to determine whether pretreatment values were associated with disease‐free interval or with ST. We hypothesized that Treg numbers would be increased in dogs with cancer and that dogs with a high percentage of Treg would have a poorer prognosis.
Animals: Twelve client‐owned dogs with appendicular OSA were entered into a prospective clinical trial. Twenty‐two healthy dogs were used as controls.
Methods: The percentages and numbers of Treg and CD4+ and CD8+ T cells in blood, lymph nodes, and tumors were determined with flow cytometry and compared between dogs with OSA and control dogs.
Results: Dogs with OSA had significantly fewer circulating CD8+ T cells and significantly more Treg compared with healthy dogs. The CD8/Treg ratio also was significantly lower in dogs with OSA compared with control dogs. In dogs with OSA, a decreased CD8/Treg ratio was associated with significantly shorter STs.
Conclusions: These data support a role for Treg in the immune control of canine OSA and suggest that determination of the CD8/Treg ratio may be useful for assessing outcomes.
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