[HTML][HTML] Differential cleavage of eIF4GI and eIF4GII in mammalian cells: effects on translation
A Castelló, E Alvarez, L Carrasco - Journal of Biological Chemistry, 2006 - ASBMB
Two isoforms of the translation initiation factor eIF4G, eIF4GI and eIF4GII, have been
described in eukaryotic cells. The exact function of each isoform during the initiation of
protein synthesis is still under investigation. We have developed an efficient and reliable
method of expressing poliovirus 2Apro, which differentially proteolyzes eIF4GI and eIF4GII in
a time-and dose-dependent manner. This system is based on the electroporation of an in
vitro transcribed mRNA that contains the encephalomyocarditis virus internal ribosome entry …
described in eukaryotic cells. The exact function of each isoform during the initiation of
protein synthesis is still under investigation. We have developed an efficient and reliable
method of expressing poliovirus 2Apro, which differentially proteolyzes eIF4GI and eIF4GII in
a time-and dose-dependent manner. This system is based on the electroporation of an in
vitro transcribed mRNA that contains the encephalomyocarditis virus internal ribosome entry …