Isolation of a new metabolite of vitamin D produced in vivo, 1α, 25-dihydroxyvitamin D3-26, 23-lactone
N Ohnuma, K Bannai, H Yamaguchi… - Archives of Biochemistry …, 1980 - Elsevier
N Ohnuma, K Bannai, H Yamaguchi, Y Hashimoto, AW Norman
Archives of Biochemistry and Biophysics, 1980•ElsevierA new vitamin D metabolite was isolated in pure form from the blood of rats given oral doses
of 50 μg/kg of 1α-hydroxyvitamin D 3. The isolation involved methanol-chloroform extraction
and four successive column chromatographic procedures. A tentative structure of the
metabolite is proposed on the basis of its column chromatographic behavior via mass
spectrometry, ultraviolet absorption spectrophotometry, and as 1α, 3β, 25-trihydroxy-9, 10
(19)-cholestatrieno-26, 23-lactone. The trivial name 1α, 25-dihydroxyvitamin D 3-26, 23 …
of 50 μg/kg of 1α-hydroxyvitamin D 3. The isolation involved methanol-chloroform extraction
and four successive column chromatographic procedures. A tentative structure of the
metabolite is proposed on the basis of its column chromatographic behavior via mass
spectrometry, ultraviolet absorption spectrophotometry, and as 1α, 3β, 25-trihydroxy-9, 10
(19)-cholestatrieno-26, 23-lactone. The trivial name 1α, 25-dihydroxyvitamin D 3-26, 23 …
Abstract
A new vitamin D metabolite was isolated in pure form from the blood of rats given oral doses of 50 μg/kg of 1α-hydroxyvitamin D3. The isolation involved methanol-chloroform extraction and four successive column chromatographic procedures. A tentative structure of the metabolite is proposed on the basis of its column chromatographic behavior via mass spectrometry, ultraviolet absorption spectrophotometry, and as 1α,3β,25-trihydroxy-9,10 (19)-cholestatrieno-26,23-lactone. The trivial name 1α,25-dihydroxyvitamin D3-26,23-lactone is suggested for this compound.
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