[HTML][HTML] Two SUR1-specific histidine residues mandatory for zinc-induced activation of the rat KATP channel
V Bancila, T Cens, D Monnier, F Chanson… - Journal of Biological …, 2005 - ASBMB
Zinc at micromolar concentrations hyperpolarizes rat pancreatic β-cells and brain nerve
terminals by activating ATP-sensitive potassium channels (K ATP). The molecular
determinants of this effect were analyzed using insulinoma cell lines and cells transfected
with either wild type or mutated K ATP subunits. Zinc activated K ATP in cells co-expressing
rat Kir6. 2 and SUR1 subunits, as in insulinoma cell lines. In contrast, zinc exerted an
inhibitory action on SUR2A-containing cells. Therefore, SUR1 expression is required for the …
terminals by activating ATP-sensitive potassium channels (K ATP). The molecular
determinants of this effect were analyzed using insulinoma cell lines and cells transfected
with either wild type or mutated K ATP subunits. Zinc activated K ATP in cells co-expressing
rat Kir6. 2 and SUR1 subunits, as in insulinoma cell lines. In contrast, zinc exerted an
inhibitory action on SUR2A-containing cells. Therefore, SUR1 expression is required for the …