Protein secondary structure analyses from circular dichroism spectroscopy: methods and reference databases

L Whitmore, BA Wallace - Biopolymers: Original Research on …, 2008 - Wiley Online Library
Biopolymers: Original Research on Biomolecules, 2008Wiley Online Library
Circular dichroism (CD) spectroscopy has been a valuable method for the analysis of protein
secondary structures for many years. With the advent of synchrotron radiation circular
dichroism (SRCD) and improvements in instrumentation for conventional CD, lower
wavelength data are obtainable and the information content of the spectra increased. In
addition, new computation and bioinformatics methods have been developed and new
reference databases have been created, which greatly improve and facilitate the analyses of …
Abstract
Circular dichroism (CD) spectroscopy has been a valuable method for the analysis of protein secondary structures for many years. With the advent of synchrotron radiation circular dichroism (SRCD) and improvements in instrumentation for conventional CD, lower wavelength data are obtainable and the information content of the spectra increased. In addition, new computation and bioinformatics methods have been developed and new reference databases have been created, which greatly improve and facilitate the analyses of CD spectra. This article discusses recent developments in the analysis of protein secondary structures, including features of the DICHROWEB analysis webserver. © 2007 Wiley Periodicals, Inc. Biopolymers 89: 392–400, 2008.
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