Dual effect of glucose on cytoplasmic Ca2+ in single pancreatic β-cells
E Grapengiesser, E Gylfe, B Hellman - Biochemical and biophysical …, 1988 - Elsevier
E Grapengiesser, E Gylfe, B Hellman
Biochemical and biophysical research communications, 1988•ElsevierAbstract The cytoplasmic Ca 2+ concentration (Ca 2+ i) was measured in single pancreatic β-
cells from ob ob-mice using the fluorescent indicator fura-2. Raising the glucose
concentration from 3 to 20 mM resulted in 25% initial lowering of Ca 2+ i, followed by 250%
rise above the basal level of 49±3 nM. Tolbutamide (100 μM) was as effective as glucose in
increasing Ca 2+ i, although its action was more rapid and not preceded by any reduction.
The results support the concept that stimulated removal of Ca 2+ from the cytoplasm is an …
cells from ob ob-mice using the fluorescent indicator fura-2. Raising the glucose
concentration from 3 to 20 mM resulted in 25% initial lowering of Ca 2+ i, followed by 250%
rise above the basal level of 49±3 nM. Tolbutamide (100 μM) was as effective as glucose in
increasing Ca 2+ i, although its action was more rapid and not preceded by any reduction.
The results support the concept that stimulated removal of Ca 2+ from the cytoplasm is an …
Abstract
Abstract The cytoplasmic Ca 2+ concentration (Ca 2+ i) was measured in single pancreatic β-cells from ob ob-mice using the fluorescent indicator fura-2. Raising the glucose concentration from 3 to 20 mM resulted in 25% initial lowering of Ca 2+ i, followed by 250% rise above the basal level of 49±3 nM. Tolbutamide (100 μM) was as effective as glucose in increasing Ca 2+ i, although its action was more rapid and not preceded by any reduction. The results support the concept that stimulated removal of Ca 2+ from the cytoplasm is an essential part of the physiological glucose effect on the pancreatic β-cells.
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