[HTML][HTML] Effects of prothrombin on the individual activated protein C-mediated cleavages of coagulation factor Va
S Tran, E Norstrøm, B Dahlback - Journal of biological chemistry, 2008 - ASBMB
The factor Va (FVa) inactivation by activated protein C (APC), mediated by cleavages at Arg
306 and Arg 506 in FVa, is inhibited by both factor Xa (FXa) and prothrombin. Although FXa
is known to specifically inhibit the Arg 506 cleavage, the effect of prothrombin has not been
confined to one cleavage site. We used recombinant FV variants, FV: R506Q/R679Q and
FV: R306Q/R679Q, to investigate the effect of prothrombin on the individual cleavage sites.
The APC-mediated FVa inhibition was monitored by a prothrombinase-based FVa assay …
306 and Arg 506 in FVa, is inhibited by both factor Xa (FXa) and prothrombin. Although FXa
is known to specifically inhibit the Arg 506 cleavage, the effect of prothrombin has not been
confined to one cleavage site. We used recombinant FV variants, FV: R506Q/R679Q and
FV: R306Q/R679Q, to investigate the effect of prothrombin on the individual cleavage sites.
The APC-mediated FVa inhibition was monitored by a prothrombinase-based FVa assay …