Parallel gene cloning and protein production in multiple expression systems
HM Wang, YP Shih, SM Hu, WT Lo… - Biotechnology …, 2009 - Wiley Online Library
Biotechnology progress, 2009•Wiley Online Library
To quickly find an optimal expression system for recombinant protein production, a set of
vectors with the same restriction sites were constructed for parallel cloning of a target gene
and recombinant protein production in prokaryotic and eukaryotic expression systems,
simultaneously. These vectors include nucleotide sequences encoding protein tags and
protease recognition sites for tag removal, followed by the cloning sites 5′‐EcoRI/3′‐XhoI
identical in these vectors for ligating with the sticky‐end PCR product of a target gene. Our …
vectors with the same restriction sites were constructed for parallel cloning of a target gene
and recombinant protein production in prokaryotic and eukaryotic expression systems,
simultaneously. These vectors include nucleotide sequences encoding protein tags and
protease recognition sites for tag removal, followed by the cloning sites 5′‐EcoRI/3′‐XhoI
identical in these vectors for ligating with the sticky‐end PCR product of a target gene. Our …
Abstract
To quickly find an optimal expression system for recombinant protein production, a set of vectors with the same restriction sites were constructed for parallel cloning of a target gene and recombinant protein production in prokaryotic and eukaryotic expression systems, simultaneously. These vectors include nucleotide sequences encoding protein tags and protease recognition sites for tag removal, followed by the cloning sites 5′‐EcoRI/3′‐XhoI identical in these vectors for ligating with the sticky‐end PCR product of a target gene. Our vectors allow parallel gene cloning and protein production in multiple expression systems with minimal cloning effort. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009
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