[HTML][HTML] HIV-1 Gag processing intermediates trans-dominantly interfere with HIV-1 infectivity

B Müller, M Anders, H Akiyama, S Welsch… - Journal of Biological …, 2009 - ASBMB
Protease inhibitors (PI) act by blocking human immunodeficiency virus (HIV) polyprotein
processing, but there is no direct quantitative correlation between the degree of impairment
of Gag processing and virion infectivity at low PI concentrations. To analyze the
consequences of partial processing, virus particles were produced in the presence of
limiting PI concentrations or by co-transfection of wild-type proviral plasmids with constructs
carrying mutations in one or more cleavage sites. Low PI concentrations caused subtle …