A highly sensitive and dynamic immunofluorescent cytometric bead assay for the detection of HIV-1 p24
A Biancotto, B Brichacek, SS Chen, W Fitzgerald… - Journal of virological …, 2009 - Elsevier
Nucleic acid measurements are used to follow HIV-1 viral load in clinical applications while
p24 ELISA is commonly used to monitor HIV-1 replication in research settings. Current
ELISA assays are expensive and offer a narrow dynamic measurement range. This report
describes a simple, sensitive and inexpensive bead-based assay offering a wide dynamic
measurement range. This cytometric bead assay allows the detection of p24 concentrations
over 4 orders of magnitude from less than 0.4 pg to up to 20,000 pgml− 1 in a volume of 50μl …
p24 ELISA is commonly used to monitor HIV-1 replication in research settings. Current
ELISA assays are expensive and offer a narrow dynamic measurement range. This report
describes a simple, sensitive and inexpensive bead-based assay offering a wide dynamic
measurement range. This cytometric bead assay allows the detection of p24 concentrations
over 4 orders of magnitude from less than 0.4 pg to up to 20,000 pgml− 1 in a volume of 50μl …