The modulation of inflammatory oedema by calcitonin gene‐related peptide
P Newbold, SD Brain - British journal of pharmacology, 1993 - Wiley Online Library
P Newbold, SD Brain
British journal of pharmacology, 1993•Wiley Online Library1 The effect of calcitonin gene‐related peptide (CGRP) when given with or as a pretreatment
to oedema inducing agents was investigated in the skin and paws of male Wistar and
Sprague Dawley rats. 2 Oedema formation at intradermally‐injected sites in the skin was
measured by a 125I‐labelled human serum albumin accumulation technique and paw
oedema was measured by a weight displacement technique. 3 CGRP (30 pmol) when given
with, or as a 20 min pretreatment, markedly potentiated oedema formation induced by …
to oedema inducing agents was investigated in the skin and paws of male Wistar and
Sprague Dawley rats. 2 Oedema formation at intradermally‐injected sites in the skin was
measured by a 125I‐labelled human serum albumin accumulation technique and paw
oedema was measured by a weight displacement technique. 3 CGRP (30 pmol) when given
with, or as a 20 min pretreatment, markedly potentiated oedema formation induced by …
- 1The effect of calcitonin gene‐related peptide (CGRP) when given with or as a pretreatment to oedema inducing agents was investigated in the skin and paws of male Wistar and Sprague Dawley rats.
- 2Oedema formation at intradermally‐injected sites in the skin was measured by a 125I‐labelled human serum albumin accumulation technique and paw oedema was measured by a weight displacement technique.
- 3CGRP (30 pmol) when given with, or as a 20 min pretreatment, markedly potentiated oedema formation induced by substance P (100 pmol) in rat skin. In comparison, CGRP had little effect on 5‐hydroxytryptamine (5‐HT, 0.1–3 nmol)‐induced oedema when given as a co‐injection but significantly potentiated 5‐HT‐induced oedema when given as a 20 min pretreatment in the skin. Similar results were obtained in both Wistar and Sprague Dawley rats.
- 4Pretreatment with CGRP (30 pmol) had little modulatory effect on oedema induced by substance P (100 pmol) in the presence of the vasodilator prostanoid, prostaglandin E1 (PGE1, 850 pmol) in the skin of Wistar rats.
- 5Pretreatment with CGRP (30 pmol) caused a non‐significant increase in carrageenin (300 μg)‐induced oedema in the hind paw of Wistar rats. Capsaicin (100 nmol) given as a pretreatment had little effect on carrageenin‐induced oedema.
- 6CGRP (30 pmol), given as a pretreatment, had little modulatory effect on 5‐HT (3 nmol)‐induced oedema in the paw of Wistar rats but a non‐significant decrease in paw oedema was observed in Sprague Dawley rats.
- 7The results suggest that CGRP, either given together with or as a pretreatment to mediators of increased microvascular permeability, depending on circumstance, can act in a synergistic manner to increase oedema formation. Little evidence was obtained for an anti‐inflammatory effect of CGRP in either the rat skin or paw.
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