Establishing intracranial brain tumor xenografts with subsequent analysis of tumor growth and response to therapy using bioluminescence imaging
T Ozawa, CD James - JoVE (Journal of Visualized Experiments), 2010 - jove.com
Transplantation models using human brain tumor cells have served an essential function in
neuro-oncology research for many years. In the past, the most commonly used procedure for
human tumor xenograft establishment consisted of the collection of cells from culture flasks,
followed by the subcutaneous injection of the collected cells in immunocompromised mice.
Whereas this approach still sees frequent use in many laboratories, there has been a
significant shift in emphasis over the past decade towards orthotopic xenograft …
neuro-oncology research for many years. In the past, the most commonly used procedure for
human tumor xenograft establishment consisted of the collection of cells from culture flasks,
followed by the subcutaneous injection of the collected cells in immunocompromised mice.
Whereas this approach still sees frequent use in many laboratories, there has been a
significant shift in emphasis over the past decade towards orthotopic xenograft …