1H and 13C NMR study of 2‐hydroxyglutaric acid and its lactone

D Bal, A Gryff‐Keller - Magnetic Resonance in Chemistry, 2002 - Wiley Online Library
D Bal, A Gryff‐Keller
Magnetic Resonance in Chemistry, 2002Wiley Online Library
Determination of the level and absolute configuration of 2‐hydroxyglutaric acid in a patient's
urine is a method of diagnosing two metabolic diseases. Such a determination can be done
with the aid of NMR spectroscopic methods. In this paper the careful interpretation of 1H and
13C NMR spectra of this metabolite and its lactone measured under conditions used in
biomedical assays is reported. The 1H chemical shifts and spin–spin coupling constants
were derived using the total lineshape analysis method. Copyright© 2002 John Wiley & …
Abstract
Determination of the level and absolute configuration of 2‐hydroxyglutaric acid in a patient's urine is a method of diagnosing two metabolic diseases. Such a determination can be done with the aid of NMR spectroscopic methods. In this paper the careful interpretation of 1H and 13C NMR spectra of this metabolite and its lactone measured under conditions used in biomedical assays is reported. The 1H chemical shifts and spin–spin coupling constants were derived using the total lineshape analysis method. Copyright © 2002 John Wiley & Sons, Ltd.
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