A serum-free method for culturing normal human bronchial epithelial cells at clonal density
JF Lechner, MA LaVeck - Journal of tissue culture methods, 1985 - Springer
JF Lechner, MA LaVeck
Journal of tissue culture methods, 1985•SpringerA technique for the isolation and culture of normal human bronchial epithelial (NHBE) cells
is described. This procedure involves first explanting fragments of large airway tissue to
initiate fibroblastic-cell-free outgrowths of epithelial cells and subsequently using a serum-
free medium (LHC-9) to propagate the NHBE cells at clonal density.
is described. This procedure involves first explanting fragments of large airway tissue to
initiate fibroblastic-cell-free outgrowths of epithelial cells and subsequently using a serum-
free medium (LHC-9) to propagate the NHBE cells at clonal density.
Summary
A technique for the isolation and culture of normal human bronchial epithelial (NHBE) cells is described. This procedure involves first explanting fragments of large airway tissue to initiate fibroblastic-cell-free outgrowths of epithelial cells and subsequently using a serum-free medium (LHC-9) to propagate the NHBE cells at clonal density.
Springer