[HTML][HTML] 1, 25-dihydroxyvitamin D3 suppresses renin gene transcription by blocking the activity of the cyclic AMP response element in the renin gene promoter
W Yuan, W Pan, J Kong, W Zheng, FL Szeto… - Journal of biological …, 2007 - ASBMB
We have shown that 1, 25-dihydroxyvitamin D 3 (1, 25 (OH) 2 D 3) down-regulates renin
expression. To explore the molecular mechanism, we analyzed the mouse Ren-1c gene
promoter by luciferase reporter assays. Deletion analysis revealed two DNA fragments from–
2725 to–2647 (distal fragment) and from–117 to+ 6 (proximal fragment) that are sufficient to
mediate the repression. Mutation of the cAMP response element (CRE) in the distal fragment
blunted forskolin stimulation as well as 1, 25 (OH) 2 D 3 inhibition of the transcriptional …
expression. To explore the molecular mechanism, we analyzed the mouse Ren-1c gene
promoter by luciferase reporter assays. Deletion analysis revealed two DNA fragments from–
2725 to–2647 (distal fragment) and from–117 to+ 6 (proximal fragment) that are sufficient to
mediate the repression. Mutation of the cAMP response element (CRE) in the distal fragment
blunted forskolin stimulation as well as 1, 25 (OH) 2 D 3 inhibition of the transcriptional …