[HTML][HTML] The recombinant expression of full-length type VII collagen and characterization of molecular mechanisms underlying dystrophic epidermolysis bullosa
M Chen, FK Costa, CR Lindvay, YP Han… - Journal of Biological …, 2002 - ASBMB
Type VII collagen is a major component of anchoring fibrils, attachment structures that
mediate dermal-epidermal adherence in human skin. Dystrophic epidermolysis bullosa
(DEB) is an inherited mechano-bullous disorder caused by mutations in the type VII collagen
gene and perturbations in anchoring fibrils. In this study, we produced recombinant human
type VII collagen in stably transfected human 293 cell clones and purified large quantities of
the recombinant protein from culture media. The recombinant type VII collagen was secreted …
mediate dermal-epidermal adherence in human skin. Dystrophic epidermolysis bullosa
(DEB) is an inherited mechano-bullous disorder caused by mutations in the type VII collagen
gene and perturbations in anchoring fibrils. In this study, we produced recombinant human
type VII collagen in stably transfected human 293 cell clones and purified large quantities of
the recombinant protein from culture media. The recombinant type VII collagen was secreted …