Two new human tumor cell lines derived from squamous cell carcinomas of the tongue: establishment, characterization and response to cytotoxic treatment
J Gioanni, JL Fischel, JC Lambert, F Demard… - European Journal of …, 1988 - Elsevier
Two new permanent cell lines derived from squamous cell carcinomas of the tongue, CAL
27 and CAL 33, have been established in culture. Both cell lines were isolated in standard
culture media without epidermal growth factor or fibroblast feeder layer to avoid obtaining
clones of more differentiated cells. Analysis of the morphology, ultrastructure, karyotype and
immunohistochemical properties of these two cell lines demonstrated that they are both well
characterized, uncontaminated by HeLa cells, and do in fact correspond to transformed …
27 and CAL 33, have been established in culture. Both cell lines were isolated in standard
culture media without epidermal growth factor or fibroblast feeder layer to avoid obtaining
clones of more differentiated cells. Analysis of the morphology, ultrastructure, karyotype and
immunohistochemical properties of these two cell lines demonstrated that they are both well
characterized, uncontaminated by HeLa cells, and do in fact correspond to transformed …