[HTML][HTML] The stability and transactivation potential of the mammalian MafA transcription factor are regulated by serine 65 phosphorylation
S Guo, R Burnette, L Zhao, NL Vanderford… - Journal of biological …, 2009 - ASBMB
The level of the MafA transcription factor is regulated by a variety of effectors of β cell
function, including glucose, fatty acids, and insulin. Here, we show that phosphorylation at
Ser 65 of mammalian MafA influences both protein stability and transactivation potential.
Replacement of Ser 65 with Glu to mimic phosphorylation produced a protein that was as
unstable as the wild type, whereas Asp or Ala mutation blocked degradation. Analysis of
MafA chimeric and deletion constructs suggests that protein phosphorylation at Ser 65 alone …
function, including glucose, fatty acids, and insulin. Here, we show that phosphorylation at
Ser 65 of mammalian MafA influences both protein stability and transactivation potential.
Replacement of Ser 65 with Glu to mimic phosphorylation produced a protein that was as
unstable as the wild type, whereas Asp or Ala mutation blocked degradation. Analysis of
MafA chimeric and deletion constructs suggests that protein phosphorylation at Ser 65 alone …