[HTML][HTML] Sialoside specificity of the siglec family assessed using novel multivalent probes: identification of potent inhibitors of myelin-associated glycoprotein
O Blixt, BE Collins, IM van den Nieuwenhof… - Journal of Biological …, 2003 - ASBMB
Ten of the 11 known human siglecs or their murine orthologs have been evaluated for their
specificity for over 25 synthetic sialosides representing most of the major sequences
terminating carbohydrate groups of glycoproteins and glycolipids. Analysis has been
performed using a novel multivalent platform comprising biotinylated sialosides bound to a
streptavidin-alkaline phosphatase conjugate. Each siglec was found to have a unique
specificity for binding 16 different sialoside-streptavidin-alkaline phosphatase probes. The …
specificity for over 25 synthetic sialosides representing most of the major sequences
terminating carbohydrate groups of glycoproteins and glycolipids. Analysis has been
performed using a novel multivalent platform comprising biotinylated sialosides bound to a
streptavidin-alkaline phosphatase conjugate. Each siglec was found to have a unique
specificity for binding 16 different sialoside-streptavidin-alkaline phosphatase probes. The …