Recent studies of the distribution of PKC isoenzymes in the mouse kidney demonstrated that PKC-α, -β_I, and -δ are expressed in intercalated cells. The purpose of this study was to identify the intercalated cell subtypes that express the different PKC isoenzymes and determine the location of the PKC isoenzymes within these cells. Adult C57BL/6 mice kidney tissues were processed for multiple-labeling immunohistochemistry. Antibodies against the vacuolar H⁺-ATPase and pendrin were used to identify intercalated cell subtypes, whereas antibodies against calbindin D_28K and aquaporin-2 (AQP2) were used to identify connecting tubule cells and principal cells of the collecting duct, respectively. Within type A intercalated cells, PKC-δ was highly expressed in the apical part of the cells, whereas immunoreactivity for both PKC-α and PKC-β_I was weak. Type B intercalated cells exhibited strong expression of PKC-α, -β_I, and -δ. PKC-α and -β_I were localized throughout the cytoplasm, whereas PKC-δ was restricted to the basal domain. Within non-A-non-B cells, immunoreactivity for both PKC-α and PKC-β_I was high in intensity and localized diffusely in the cytoplasm, whereas PKC-δ was localized in the apical part of the cells. None of the PKC isoenzymes (PKC-α, -β_I, or -δ) were expressed in the calbindin D_28K-positive connecting tubule cells. Within AQP2-positive principal cells of the collecting duct, PKC-α was expressed on the basolateral plasma membrane, but no significant staining was detected for PKC-β_I and -δ. In summary, this study demonstrates distinct and differential expression patterns of PKC-α, -β_I, and -δ in the three subtypes of intercalated cells in the mouse kidney.