Isotopomer studies of gluconeogenesis and the Krebs cycle with 13C-labeled lactate.
J Katz, P Wals, WN Lee - Journal of Biological Chemistry, 1993 - ASBMB
Fasted rats were intragastrically infused with either [2, 3-13C] lactate or [1, 2, 3-13C] lactate.
The infusate also contained 14C-labeled lactate and [3-3H] glucose. Glucose, alanine,
glutamate, and glutamine were isolated from liver and blood. There was near complete
equilibration of lactate and alanine, and the relative specific activities and relative
enrichments were the same in blood and liver. Glucose was cleaved enzymatically to
lactate. The compounds were examined by gas chromatography-mass spectroscopy. From …
The infusate also contained 14C-labeled lactate and [3-3H] glucose. Glucose, alanine,
glutamate, and glutamine were isolated from liver and blood. There was near complete
equilibration of lactate and alanine, and the relative specific activities and relative
enrichments were the same in blood and liver. Glucose was cleaved enzymatically to
lactate. The compounds were examined by gas chromatography-mass spectroscopy. From …