Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase
L Kjeldsen, AH Johnsen, H Sengeløv… - Journal of Biological …, 1993 - ASBMB
A 25-kDa protein was found to be associated with purified human neutrophil gelatinase.
Polyclonal antibodies raised against gelatinase not only recognized gelatinase but also this
25-kDa protein. Specific antibodies against the 25-kDa protein were obtained by affinity
purification of the gelatinase antibodies. Immunoblotting and immunoprecipitation studies
demonstrated the 135-kDa form of gelatinase to be a complex of 92-kDa gelatinase and the
25-kDa protein, and the 220-kDa form was demonstrated to be a homodimer of the 92-kDa …
Polyclonal antibodies raised against gelatinase not only recognized gelatinase but also this
25-kDa protein. Specific antibodies against the 25-kDa protein were obtained by affinity
purification of the gelatinase antibodies. Immunoblotting and immunoprecipitation studies
demonstrated the 135-kDa form of gelatinase to be a complex of 92-kDa gelatinase and the
25-kDa protein, and the 220-kDa form was demonstrated to be a homodimer of the 92-kDa …
