Desmin is a smooth muscle protein consisting of intermediate filaments. It is also present in
other cell types. We obtained desmin antibodies from New Zealand white rabbits which were
immunized with desmin following its extraction from chicken gizzard smooth muscle. By
immunocytochemical methods, the antibody stained the Z-line of rat skeletal myofibrils and
produced only one band with chicken gizzard desmin by immunoblotting. The antibody was
specific for desmin, but was not tissue-specific. Wistar rat liver was studied by indirect …