The distribution of apolipoprotein (apo) E in rat hepatocytes was investigated with an affinity-
purified polyclonal antibody raised against apoE isolated from hepatogeneous very low
density lipoproteins (VLDL). The distribution of this antibody was visualized with colloidal
gold complexed to anti-rabbit IgG. By epipolarization microscopy, apoE was found uniformly
along the basolateral surfaces of all hepatic parenchymal cells, showing a striking intensity
along the sinusoidal front. Punctate deposits of colloidal gold appeared to be randomly …