A routine method for the establishment of permanent growing lymphoblastoid cell lines

H Neitzel - Human genetics, 1986 - Springer
H Neitzel
Human genetics, 1986Springer
Permanent lymphoblastoid cell lines are of great practical value in human clinical and
experimental genetics. A detailed protocol for routine use is given for the establishment of
lymphoblastoid lines from peripheral blood using Esptein-Barr virus and the
immunosuppressivum Cyclosporin A. In addition, the biologic basis of this transformation
system is briefly summarized.
Summary
Permanent lymphoblastoid cell lines are of great practical value in human clinical and experimental genetics. A detailed protocol for routine use is given for the establishment of lymphoblastoid lines from peripheral blood using Esptein-Barr virus and the immunosuppressivum Cyclosporin A. In addition, the biologic basis of this transformation system is briefly summarized.
Springer