SPAK-knockout mice manifest Gitelman syndrome and impaired vasoconstriction
SS Yang, YF Lo, CC Wu, SW Lin, CJ Yeh… - Journal of the …, 2010 - journals.lww.com
Polymorphisms in the gene encoding sterile 20/SPS1-related proline/alanine-rich kinase
(SPAK) associate with hypertension susceptibility in humans. SPAK interacts with WNK
kinases to regulate the Na+-K+-2Cl− and Na+-Cl− co-transporters [collectively, N (K) CC].
Mutations in WNK1/4 and N (K) CC can cause changes in BP and dyskalemia in humans,
but the physiologic role of SPAK in vivo is unknown. We generated and analyzed SPAK-null
mice by targeting disruption of exons 9 and 10 of SPAK. Compared with SPAK+/+ …
(SPAK) associate with hypertension susceptibility in humans. SPAK interacts with WNK
kinases to regulate the Na+-K+-2Cl− and Na+-Cl− co-transporters [collectively, N (K) CC].
Mutations in WNK1/4 and N (K) CC can cause changes in BP and dyskalemia in humans,
but the physiologic role of SPAK in vivo is unknown. We generated and analyzed SPAK-null
mice by targeting disruption of exons 9 and 10 of SPAK. Compared with SPAK+/+ …