[PDF][PDF] ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity

T Ohta, JJ Michel, AJ Schottelius, Y Xiong - Molecular cell, 1999 - cell.com
T Ohta, JJ Michel, AJ Schottelius, Y Xiong
Molecular cell, 1999cell.com
We have identified two highly conserved RING finger proteins, ROC1 and ROC2, that are
homologous to APC11, a subunit of the anaphase-promoting complex. ROC1 and ROC2
commonly interact with all cullins while APC11 specifically interacts with APC2, a cullin-
related APC subunit. Yeast ROC1 encodes an essential gene whose reduced expression
resulted in multiple, elongated buds and accumulation of Sic1p and Cln2p. ROC1 and
APC11 immunocomplexes can catalyze isopeptide ligations to form polyubiquitin chains in …
Abstract
We have identified two highly conserved RING finger proteins, ROC1 and ROC2, that are homologous to APC11, a subunit of the anaphase-promoting complex. ROC1 and ROC2 commonly interact with all cullins while APC11 specifically interacts with APC2, a cullin-related APC subunit. Yeast ROC1 encodes an essential gene whose reduced expression resulted in multiple, elongated buds and accumulation of Sic1p and Cln2p. ROC1 and APC11 immunocomplexes can catalyze isopeptide ligations to form polyubiquitin chains in an E1- and E2-dependent manner. ROC1 mutations completely abolished their ligase activity without noticeable changes in associated proteins. Ubiquitination of phosphorylated IκBα can be catalyzed by the ROC1 immunocomplex in vitro. Hence, combinations of ROC/APC11 and cullin proteins potentially constitute a wide variety of ubiquitin ligases.
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