[HTML][HTML] Inhibition of matrix metalloproteinase-9 by interferons and TGF-β1 through distinct signalings accounts for reduced monocyte invasiveness
J Nguyen, P Knapnougel, P Lesavre, B Bauvois - FEBS letters, 2005 - Elsevier
J Nguyen, P Knapnougel, P Lesavre, B Bauvois
FEBS letters, 2005•ElsevierCytokines may provide signals for regulating human monocyte matrix metalloproteinase-9
(MMP-9) activity. In this study, we investigated the roles of interferons (IFN) type I/II and
transforming growth factor-β1 (TGF-β1) in MMP-9-mediated invasiveness. MMP-9 antibody
and inhibitor, IFNs and TGF-β1 inhibited monocyte transmigration through Matrigel. IFNs
and TGF-β1 downregulated MMP-9 mRNA, protein and activity levels. The inhibitory action
of IFNs was associated with the STAT1/IRF-1 pathway since the JAK inhibitor AG490 …
(MMP-9) activity. In this study, we investigated the roles of interferons (IFN) type I/II and
transforming growth factor-β1 (TGF-β1) in MMP-9-mediated invasiveness. MMP-9 antibody
and inhibitor, IFNs and TGF-β1 inhibited monocyte transmigration through Matrigel. IFNs
and TGF-β1 downregulated MMP-9 mRNA, protein and activity levels. The inhibitory action
of IFNs was associated with the STAT1/IRF-1 pathway since the JAK inhibitor AG490 …
Cytokines may provide signals for regulating human monocyte matrix metalloproteinase-9 (MMP-9) activity. In this study, we investigated the roles of interferons (IFN) type I/II and transforming growth factor-β1 (TGF-β1) in MMP-9-mediated invasiveness. MMP-9 antibody and inhibitor, IFNs and TGF-β1 inhibited monocyte transmigration through Matrigel. IFNs and TGF-β1 downregulated MMP-9 mRNA, protein and activity levels. The inhibitory action of IFNs was associated with the STAT1/IRF-1 pathway since the JAK inhibitor AG490 blocked STAT1 phosphorylation, IRF-1 synthesis and counteracted the blockade of MMP-9 release. TGF-β1-mediated MMP-9 inhibition appeared STAT1/IRF-1-independent but reversed by the protein tyrosine kinase inhibitor tyrphostin 25. Our data point out the importance of IFNs and TGF-β1 in the control of monocyte MMP-9-mediated extravasation.
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