In vitro propagation of human prepubertal spermatogonial stem cells
H Sadri-Ardekani, MA Akhondi, F van der Veen… - Jama, 2011 - jamanetwork.com
Results. Two and a half weeks after initiation of the testicular cell culture, the first germline
stem cell (GSC) clusters appeared. Testicular cells were cultured for 20 and 15.5 weeks from
the 6.5-and 8-year-old boys, respectively. GSC clusters were subcultured on laminin for a
total of 29 and 20 weeks from the 6.5-and 8-year-old boys, respectively. Expression of
spermatogonial markers was detected throughout the entire culture period at the RNA
(FIGURE 1) and protein levels (ZBTB16 and UCHL1). Eight weeks after xenotransplantation …
stem cell (GSC) clusters appeared. Testicular cells were cultured for 20 and 15.5 weeks from
the 6.5-and 8-year-old boys, respectively. GSC clusters were subcultured on laminin for a
total of 29 and 20 weeks from the 6.5-and 8-year-old boys, respectively. Expression of
spermatogonial markers was detected throughout the entire culture period at the RNA
(FIGURE 1) and protein levels (ZBTB16 and UCHL1). Eight weeks after xenotransplantation …