Characterization of serine palmitoyltransferase activity in Chinese hamster ovary cells
AH Merrill Jr - Biochimica et Biophysica Acta (BBA)-Lipids and Lipid …, 1983 - Elsevier
Serine palmitoyltansferase (palmitoyl-CoA: l-serine C-palmitoyltransferase (decarboxylating)
EC 2.3. 1.50) catalyzes the first unique and regulatory reaction of sphingolipid biosynthesis.
Its activity was demonstrated in Chinese hamster ovary cells (CHO-K1) by measuring the
incorporation of radiolabel from l-[3 H] serine into 3-ketosphinganine, which was found to be
the predominant chloroform-soluble product under optimal assay conditions. Most of the
total activity (14.8±4.2 pmol/min per 10 6 cells, measured with sonicated cells) was …
EC 2.3. 1.50) catalyzes the first unique and regulatory reaction of sphingolipid biosynthesis.
Its activity was demonstrated in Chinese hamster ovary cells (CHO-K1) by measuring the
incorporation of radiolabel from l-[3 H] serine into 3-ketosphinganine, which was found to be
the predominant chloroform-soluble product under optimal assay conditions. Most of the
total activity (14.8±4.2 pmol/min per 10 6 cells, measured with sonicated cells) was …