Genome-wide screening for complete genetic loss in prostate cancer by comparative hybridization onto cDNA microarrays
J Clark, S Edwards, A Feber, P Flohr, M John… - Oncogene, 2003 - nature.com
J Clark, S Edwards, A Feber, P Flohr, M John, I Giddings, S Crossland, MR Stratton…
Oncogene, 2003•nature.comWe demonstrate that comparative genomic hybridization (CGH) onto cDNA microarrays may
be used to carry out genome-wide screens for regions of genetic loss, including
homozygous (complete) deletions that may represent the possible location of tumour
suppressor genes in human cancer. Screening of the prostate cancer cell lines LNCaP, PC3
and DU145 allowed the mapping of specific regions where genome copy number appeared
altered and led to the identification of two novel regions of complete loss at 17q21. 31 (500 …
be used to carry out genome-wide screens for regions of genetic loss, including
homozygous (complete) deletions that may represent the possible location of tumour
suppressor genes in human cancer. Screening of the prostate cancer cell lines LNCaP, PC3
and DU145 allowed the mapping of specific regions where genome copy number appeared
altered and led to the identification of two novel regions of complete loss at 17q21. 31 (500 …
Abstract
We demonstrate that comparative genomic hybridization (CGH) onto cDNA microarrays may be used to carry out genome-wide screens for regions of genetic loss, including homozygous (complete) deletions that may represent the possible location of tumour suppressor genes in human cancer. Screening of the prostate cancer cell lines LNCaP, PC3 and DU145 allowed the mapping of specific regions where genome copy number appeared altered and led to the identification of two novel regions of complete loss at 17q21. 31 (500 kb spanning STAT3) and at 10q23. 1 (50–350 kb spanning SFTPA2) in the PC3 cell line.
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