Complex formation of JAK2 with PP2A, PI3K, and Yes in response to the hematopoietic cytokine interleukin-11

DK Fuhrer, YC Yang - Biochemical and biophysical research …, 1996 - Elsevier
DK Fuhrer, YC Yang
Biochemical and biophysical research communications, 1996Elsevier
Interleukin-11 is a stromal derived cytokine important in hematopoiesis. IL-11 intracellular
signaling travels through cytoplasmic kinases of the Janus family. How JAKs accomplish the
multiple functions of IL-11 has not been determined and until recently only a few associated
downstream proteins have been identified. We present evidence here for the IL-11 induced
association of PP2A, PI3K, and Yes to JAK2. Reciprocal immunoprecipitations support the
mutual involvement of these signaling components in IL-11 mediated signal transduction …
Interleukin-11 is a stromal derived cytokine important in hematopoiesis. IL-11 intracellular signaling travels through cytoplasmic kinases of the Janus family. How JAKs accomplish the multiple functions of IL-11 has not been determined and until recently only a few associated downstream proteins have been identified. We present evidence here for the IL-11 induced association of PP2A, PI3K, and Yes to JAK2. Reciprocal immunoprecipitations support the mutual involvement of these signaling components in IL-11 mediated signal transduction. This novel finding of JAK2/PP2A binding and release may have relevance to many serine/threonine regulated mechanisms such as PI3K, Stat, and MAPK activation. These associations support a model of JAK2 as a protein kinase docking protein of IL-11 signal transduction that may be applicable to other gp130 and JAK signal transduction systems.
Elsevier