[HTML][HTML] RGS4 is arginylated and degraded by the N-end rule pathway in vitro
IV Davydov, A Varshavsky - Journal of Biological Chemistry, 2000 - ASBMB
The N-end rule relates the in vivohalf-life of a protein to the identity of its N-terminal residue.
We used an expression-cloning screen to search for mouse proteins that are degraded by
the ubiquitin/proteasome-dependent N-end rule pathway in a reticulocyte lysate. One
substrate thus identified was RGS4, a member of the RGS family of GTPase-activating
proteins that down-regulate specific G proteins. A determinant of the RGS4 degradation
signal (degron) was located at the N terminus of RGS4, because converting cysteine 2 to …
We used an expression-cloning screen to search for mouse proteins that are degraded by
the ubiquitin/proteasome-dependent N-end rule pathway in a reticulocyte lysate. One
substrate thus identified was RGS4, a member of the RGS family of GTPase-activating
proteins that down-regulate specific G proteins. A determinant of the RGS4 degradation
signal (degron) was located at the N terminus of RGS4, because converting cysteine 2 to …