[HTML][HTML] Differential sensitivity of phosphatidylinositol 3-kinase p110γ to isoforms of G protein βγ dimers
KR Kerchner, RL Clay, G McCleery, N Watson… - Journal of Biological …, 2004 - ASBMB
The ability of G protein α and βγ subunits to activate the p110γ isoform of
phosphatidylinositol 3-kinase (PtdIns 3-kinase) was examined using pure, recombinant G
proteins and the p101/p110γ form of PtdIns 3-kinase reconstituted into synthetic lipid
vesicles. GTP-activated G s, G i, G q, or G o α subunits were unable to activate PtdIns 3-
kinase. Dimers containing Gβ 1–4 complexed with γ 2-stimulated PtdIns 3-kinase activity
about 26-fold with EC 50 values ranging from 4 to 7 nm. Gβ 5 γ 2 was not able to stimulate …
phosphatidylinositol 3-kinase (PtdIns 3-kinase) was examined using pure, recombinant G
proteins and the p101/p110γ form of PtdIns 3-kinase reconstituted into synthetic lipid
vesicles. GTP-activated G s, G i, G q, or G o α subunits were unable to activate PtdIns 3-
kinase. Dimers containing Gβ 1–4 complexed with γ 2-stimulated PtdIns 3-kinase activity
about 26-fold with EC 50 values ranging from 4 to 7 nm. Gβ 5 γ 2 was not able to stimulate …