NKX2-5eGFP/w hESCs for isolation of human cardiac progenitors and cardiomyocytes
DA Elliott, SR Braam, K Koutsis, ES Ng, R Jenny… - Nature …, 2011 - nature.com
DA Elliott, SR Braam, K Koutsis, ES Ng, R Jenny, EL Lagerqvist, C Biben, T Hatzistavrou…
Nature methods, 2011•nature.comNKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-
5 locus of human embryonic stem cells (hESCs); NKX2-5 eGFP/w hESCs facilitate
quantification of cardiac differentiation, purification of hESC-derived committed cardiac
progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of
differentiation protocols. We used NKX2-5 eGFP+ cells to identify VCAM1 and SIRPA as cell-
surface markers expressed in cardiac lineages.
5 locus of human embryonic stem cells (hESCs); NKX2-5 eGFP/w hESCs facilitate
quantification of cardiac differentiation, purification of hESC-derived committed cardiac
progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of
differentiation protocols. We used NKX2-5 eGFP+ cells to identify VCAM1 and SIRPA as cell-
surface markers expressed in cardiac lineages.
Abstract
NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-5eGFP/w hESCs facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of differentiation protocols. We used NKX2-5 eGFP+ cells to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages.
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