The effect of growth conditions on NAD+ and NADH concentrations and the NAD+: NADH ratio in normal and transformed fibroblasts
JP Schwartz, JV Passonneau, GS Johnson… - Journal of Biological …, 1974 - ASBMB
Concentrations of total NAD+ and NADH have been measured and the NAD+: NADH ratio
calculated for 3T3 fibroblasts and SV40 transformed 3T3 cells and for normal rat kidney
fibroblasts and murine sarcoma virus (Kirsten strain)-transformed normal rat kidney
fibroblasts. The absolute levels were found to decrease 2-to 3-fold with transformation. The
NAD+: NADH ratio in the normal cells ranged from 3.1 to 3.6, and increased 3-to 4-fold as
they grew from logarithmic to stationary phase. The final ratio was found to be a direct …
calculated for 3T3 fibroblasts and SV40 transformed 3T3 cells and for normal rat kidney
fibroblasts and murine sarcoma virus (Kirsten strain)-transformed normal rat kidney
fibroblasts. The absolute levels were found to decrease 2-to 3-fold with transformation. The
NAD+: NADH ratio in the normal cells ranged from 3.1 to 3.6, and increased 3-to 4-fold as
they grew from logarithmic to stationary phase. The final ratio was found to be a direct …