Separation of adeno-associated virus type 2 empty particles from genome containing vectors by anion-exchange column chromatography
G Qu, J Bahr-Davidson, J Prado, A Tai… - Journal of virological …, 2007 - Elsevier
Adeno-associated virus (AAV) empty capsids typically co-purify with genome containing
AAV2 vectors purified by column chromatography. This study describes a method to remove
empty capsids from genome containing vector particles by anion exchange chromatography.
The separation is based on the slightly less anionic character of empty particles compared to
vectors. Detailed methods to achieve AAV2 vector purification and particle separation using
cation exchange resin POROS 50HS followed by anion exchange resin Q-Sepharosexl are …
AAV2 vectors purified by column chromatography. This study describes a method to remove
empty capsids from genome containing vector particles by anion exchange chromatography.
The separation is based on the slightly less anionic character of empty particles compared to
vectors. Detailed methods to achieve AAV2 vector purification and particle separation using
cation exchange resin POROS 50HS followed by anion exchange resin Q-Sepharosexl are …