[HTML][HTML] The proline-rich domain of dynamin-2 is responsible for dynamin-dependent in vitro potentiation of endothelial nitric-oxide synthase activity via selective …
S Cao, J Yao, V Shah - Journal of Biological Chemistry, 2003 - ASBMB
The GTPase dynamin-2 (dyn-2) binds and positively regulates the nitric oxide-generating
enzyme, endothelial nitric-oxide synthase (eNOS)(Cao, S., Yao, Y., McCabe, T., Yao, Q.,
Katusic, Z., Sessa, W., and Shah, V.(2001) J. Biol. Chem. 276, 14249–14256). Here we
demonstrate, using purified proteins, that this occurs through a selective influence of the dyn-
2 proline-rich domain (dyn-2 PRD) on the eNOS reductase domain. In vitro studies
demonstrate that dyn-2 PRD fused with glutathione S-transferase (GST) binds recombinant …
enzyme, endothelial nitric-oxide synthase (eNOS)(Cao, S., Yao, Y., McCabe, T., Yao, Q.,
Katusic, Z., Sessa, W., and Shah, V.(2001) J. Biol. Chem. 276, 14249–14256). Here we
demonstrate, using purified proteins, that this occurs through a selective influence of the dyn-
2 proline-rich domain (dyn-2 PRD) on the eNOS reductase domain. In vitro studies
demonstrate that dyn-2 PRD fused with glutathione S-transferase (GST) binds recombinant …