[PDF][PDF] Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes

DJ Klionsky, H Abeliovich, P Agostinis, DK Agrawal… - Autophagy, 2008 - Taylor & Francis
DJ Klionsky, H Abeliovich, P Agostinis, DK Agrawal, G Aliev, DS Askew, M Baba…
Autophagy, 2008Taylor & Francis
Research in autophagy continues to accelerate, 1 and as a result many new scientists are
entering the field. Accordingly, it is important to establish a standard set of criteria for
monitoring macroautophagy in different organisms. Recent reviews have described the
range of assays that have been used for this purpose. 2, 3 There are many useful and
convenient methods that can be used to monitor macroautophagy in yeast, but relatively few
in other model systems, and there is much confusion regarding acceptable methods to …
Research in autophagy continues to accelerate, 1 and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose. 2, 3 There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and
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