Altered expression of MiR-148a and MiR-152 in gastrointestinal cancers and its clinical significance

Y Chen, Y Song, Z Wang, Z Yue, H Xu, C Xing… - Journal of gastrointestinal …, 2010 - Elsevier
Background MicroRNAs are endogenous small noncoding RNAs that aberrantly expressed
in various carcinomas. MiR-148a and miR-152, which have the same “seed region”, have
not been comprehensively investigated in gastrointestinal cancers. Methods Total RNA was
extracted from the tissues of 101 patients with gastric cancer and 101 patients with colorectal
cancer as well as their matched nontumor adjacent tissues. After polyadenylation and
reverse transcription, the expression of miR-148a and miR-152 was determined using …