[HTML][HTML] Hypoxia-induced acidosis uncouples the STIM-Orai calcium signaling complex
S Mancarella, Y Wang, X Deng, G Landesberg… - Journal of Biological …, 2011 - ASBMB
The endoplasmic reticulum Ca 2+-sensing STIM proteins mediate Ca 2+ entry signals by
coupling to activate plasma membrane Orai channels. We reveal that STIM-Orai coupling is
rapidly blocked by hypoxia and the ensuing decrease in cytosolic pH. In smooth muscle
cells or HEK293 cells coexpressing STIM1 and Orai1, acute hypoxic conditions rapidly
blocked store-operated Ca 2+ entry and the Orai1-mediated Ca 2+ release-activated Ca 2+
current (I CRAC). Hypoxia-induced blockade of Ca 2+ entry and I CRAC was reversed by …
coupling to activate plasma membrane Orai channels. We reveal that STIM-Orai coupling is
rapidly blocked by hypoxia and the ensuing decrease in cytosolic pH. In smooth muscle
cells or HEK293 cells coexpressing STIM1 and Orai1, acute hypoxic conditions rapidly
blocked store-operated Ca 2+ entry and the Orai1-mediated Ca 2+ release-activated Ca 2+
current (I CRAC). Hypoxia-induced blockade of Ca 2+ entry and I CRAC was reversed by …