[HTML][HTML] Maturation of the epithelial Na+ channel involves proteolytic processing of the α-and γ-subunits
RP Hughey, GM Mueller, JB Bruns, CL Kinlough… - Journal of Biological …, 2003 - ASBMB
The epithelial Na+ channel (ENaC) is a tetramer of two α-, one β-, and one γ-subunit, but
little is known about its assembly and processing. Because co-expression of mouse ENaC
subunits with three different carboxyl-terminal epitope tags produced an amiloride-sensitive
sodium current in oocytes, these tagged subunits were expressed in both Chinese hamster
ovary or Madin-Darby canine kidney type 1 epithelial cells for further study. When expressed
alone α-(95 kDa), β-(96 kDa), and γ-subunits (93 kDa) each produced a single band on SDS …
little is known about its assembly and processing. Because co-expression of mouse ENaC
subunits with three different carboxyl-terminal epitope tags produced an amiloride-sensitive
sodium current in oocytes, these tagged subunits were expressed in both Chinese hamster
ovary or Madin-Darby canine kidney type 1 epithelial cells for further study. When expressed
alone α-(95 kDa), β-(96 kDa), and γ-subunits (93 kDa) each produced a single band on SDS …