[HTML][HTML] Tyrosine phosphorylation of the β4 integrin cytoplasmic domain mediates Shc signaling to extracellular signal-regulated kinase and antagonizes formation of …
M Dans, L Gagnoux-Palacios, P Blaikie, S Klein… - Journal of Biological …, 2001 - ASBMB
Ligation of the α 6 β 4 integrin induces tyrosine phosphorylation of the β 4 cytoplasmic
domain, followed by recruitment of the adaptor protein Shc and activation of mitogen-
activated protein kinase cascades. We have used Far Western analysis and
phosphopeptide competition assays to map the sites in the cytoplasmic domain of β 4 that
are required for interaction with Shc. Our results indicate that, upon phosphorylation, Tyr
1440, or secondarily Tyr 1422, interacts with the SH2 domain of Shc, whereas Tyr 1526, or …
domain, followed by recruitment of the adaptor protein Shc and activation of mitogen-
activated protein kinase cascades. We have used Far Western analysis and
phosphopeptide competition assays to map the sites in the cytoplasmic domain of β 4 that
are required for interaction with Shc. Our results indicate that, upon phosphorylation, Tyr
1440, or secondarily Tyr 1422, interacts with the SH2 domain of Shc, whereas Tyr 1526, or …