[HTML][HTML] Structure and Function in Rhodopsin: MEASUREMENT OF THE RATE OF METARHODOPSIN II DECAY BY FLUORESCENCE SPECTROSCOPY (∗)
DL Farrens, HG Khorana - Journal of Biological Chemistry, 1995 - ASBMB
An increase in fluorescence is observed upon light activation of bovine rhodopsin. The rate
of increase is monoexponential (t 1/2= 15.5 min) at 20° C in 0.1% lauryl maltoside, pH 6.0,
and parallels the rate of decay of metarhodopsin II. We show that the increase in
fluorescence is due to the release of free retinal, which no longer quenches the tryptophan
fluorescence. An extrinsic fluorescence reporter group, pyrene maleimide, attached to
bovine rhodopsin also shows an increase in pyrene fluorescence on illumination. The rate of …
of increase is monoexponential (t 1/2= 15.5 min) at 20° C in 0.1% lauryl maltoside, pH 6.0,
and parallels the rate of decay of metarhodopsin II. We show that the increase in
fluorescence is due to the release of free retinal, which no longer quenches the tryptophan
fluorescence. An extrinsic fluorescence reporter group, pyrene maleimide, attached to
bovine rhodopsin also shows an increase in pyrene fluorescence on illumination. The rate of …