[HTML][HTML] A proteomic analysis of lysosomal integral membrane proteins reveals the diverse composition of the organelle
RD Bagshaw, DJ Mahuran, JW Callahan - Molecular & Cellular Proteomics, 2005 - ASBMB
Lysosomes are endocytic subcellular compartments that contribute to the degradation and
recycling of cellular material. Using highly purified rat liver tritosomes (Triton WR1339-filled
lysosomes) and an ion exchange chromatography/LC-tandem MS-based protein/peptide
separation and identification procedure, we characterized the major integral membrane
protein complement of this organelle. While many of the 215 proteins we identified have
been previously associated with lysosomes and endosomes, others have been associated …
recycling of cellular material. Using highly purified rat liver tritosomes (Triton WR1339-filled
lysosomes) and an ion exchange chromatography/LC-tandem MS-based protein/peptide
separation and identification procedure, we characterized the major integral membrane
protein complement of this organelle. While many of the 215 proteins we identified have
been previously associated with lysosomes and endosomes, others have been associated …