[HTML][HTML] Identification of enolase as a laminin-binding protein on the surface of Staphylococcus aureus
CRW Carneiro, E Postol, R Nomizo, LFL Reis… - Microbes and …, 2004 - Elsevier
We have previously demonstrated that Staphylococcus aureus, a highly invasive bacteria,
presents a 52-kDa surface protein that mediates its binding to laminin. In order to better
characterize this receptor, we excised this putative laminin receptor from two-dimensional (2-
D) PAGE and used it as antigen for raising a mouse hyperimmune serum which was for
screening an S. aureus expression library. A single clone of 0.3 kb was obtained, and its
sequence revealed 100% homology with S. aureus α-enolase. Moreover, amino acid …
presents a 52-kDa surface protein that mediates its binding to laminin. In order to better
characterize this receptor, we excised this putative laminin receptor from two-dimensional (2-
D) PAGE and used it as antigen for raising a mouse hyperimmune serum which was for
screening an S. aureus expression library. A single clone of 0.3 kb was obtained, and its
sequence revealed 100% homology with S. aureus α-enolase. Moreover, amino acid …